Part:BBa_K5005013:Design

It is a genetically encoded NAD+ biosensor.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1200
  Illegal NgoMIV site found at 1530
  Illegal NgoMIV site found at 2034
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1495
  

Design Notes

The FiNad biosensor is employed as an NAD+ biosensor for real-time monitoring of cellular NAD+ level fluctuations. By synthesizing the mCherry-FiNad nucleotide sequence, it can be ingeniously ligated to the pCDH vector for achieving stable expression in mammalian cells. This allows real-time monitoring of dynamic NAD+ fluctuations in live cells.

Source

Constructed in this work.

References

Zou, Y., Wang, A., Huang, L., Zhu, X., Hu, Q., Zhang, Y., Chen, X., Li, F., Wang, Q., Wang, H., Liu, R., Zuo, F., Li, T., Yao, J., Qian, Y., Shi, M., Yue, X., Chen, W., Zhang, Z., Wang, C., … Zhao, Y. (2020). Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor. Developmental cell, 53(2), 240–252.e7. https://doi.org/10.1016/j.devcel.2020.02.017